affinity chromatography principles and methods by GE HEalthcare PDF

By GE HEalthcare

Show description

Read or Download affinity chromatography principles and methods PDF

Best chemistry books

Download e-book for iPad: Groundwater Geochemistry by Broder J. Merkel, Britta Planer-Friedrich, Darrell K.

The second version of "Groundwater Geochemistry – a pragmatic consultant to modeling of ordinary and infected aquatic structures" continues to be the great textual content publication providing novices and complex modellers alike a minimal theoretical heritage and a powerful specialise in the sensible resolution of geochemical modeling with PHREEQC.

Get Alkaloids: Chemical and Biological Perspectives, Vol. 11 PDF

Quantity eleven of this sequence offers 5 well timed experiences on present study on alkaloids. bankruptcy 1 by way of Paul L. Schiff, Jr. is a huge survey of analysis that has been performed during the last decade at the Thalictrum alkaloids. Forty-six new alkaloids are defined from fifteen species of the genus Thalictrum, in addition to 116 alkaloids of identified constitution from thirty-six species and subspecies of the genus.

Download e-book for iPad: The Chemistry of Haloketones, Haloaldehydes and Haloimines by Norbert; Verhe, Roland De Kimpe

This can be the 1st identify to be released within the new "Updates from the Chemistry of practical teams" sequence. those Updates can be in line with chosen chapters from the volumes of "The Chemistry of useful teams" sequence, making very important chapters to be had to the person. the chosen chapters could be taken as they stand within the unique quantity and up-to-date in an appendix showing on the finish of the textual content.

Chemistry of Heterocyclic Compounds: Heterocyclic Systems - download pdf or read online

Content material: bankruptcy I Fused three? Membered Ring platforms (pages 7–34): bankruptcy II Fused four? Membered Ring platforms (pages 35–62): bankruptcy III Fused 5/5 Ring platforms (pages 63–237): bankruptcy IV Fused 5/6 Ring platforms without additional Heteroatom (pages 239–449): bankruptcy V Fused 5/6 Ring structures with one additional Heteroatom (pages 451–747):

Additional info for affinity chromatography principles and methods

Example text

Lane 6. Lane 7. Lane 8. 75 Start refolding fr. fr. fr. 25 fr. 46 fr. 49 Start elution Manually using a syringe: • Sample loading • Gua-HCl wash • Urea wash 0 10 20 30 40 50 60 65 ml Low Molecular Weight Calibration Kit (LMW) Starting material for Lane 1. LMW HiTrap Chelating HP, 1 ml Lane 2. Fraction 38 Mr Fraction 1 Gua-HCl Lane 3. Fraction 39 wash (manually) Lane 4. Fraction 40 97 000 Fraction 2 Gua-HCl 66 000 Lane 5. Fraction 41 wash (manually) 45 000 Lane 6. Fraction 42 Fraction 3 Gua-HCl 30 000 Lane 7.

Pool I, purified Protein A-(HisGly)4His, reduced Fig. 25. Purification of recombinant proteins on HiTrap Chelating HP, 5 ml, charged with Zn2+. Recombinant protein expressed in inclusion bodies Sample: 8 ml cell extract containing (His)10-tagged protein. The clone was a kind gift from Dr. C. Fuller and S. Brasher, Department of Biochemistry, University of Cambridge, UK. 4 Flow: Approx. 4 ml/min Equipment: Syringe Electrophoresis: SDS-PAGE, PhastSystem, PhastGel 10–15, 1 µl sample, silver staining Purification in 8 M urea Mr 97 000 66 000 45 000 30 000 20 100 14 400 1 2 3 4 5 6 7 Lane 1.

Protein A Sepharose 4 Fast Flow and rProtein A Sepharose 4 Fast Flow have a higher binding capacity, a more rigid matrix and provide more convenient alternatives to Protein A Sepharose CL-4B which must be rehydrated before column packing. Chemical stability These media and columns tolerate high concentrations of urea, guanidine HCl and chaotropic agents. Storage Wash media and columns with 20% ethanol (use approximately 5 column volumes for packed media) and store at +4 to +8 °C. 37 Monoclonal IgM from hybridoma cell culture HiTrap IgM Purification HP The technique described here is optimized for purification of monoclonal IgM from hybridoma cell culture, but it can be used as a starting point to determine the binding and elution conditions required for other IgM preparations.

Download PDF sample

affinity chromatography principles and methods by GE HEalthcare

by Edward

Rated 4.89 of 5 – based on 8 votes