By Gianfranco Donelli (eds.)
The sequence will supply microbiologists, hygienists, epidemiologists and infectious illnesses experts with well-chosen contributed volumes containing up to date info within the parts of easy and utilized microbiology concerning appropriate matters for public future health, together with bacterial, fungal and parasitic infections, zoonosis and anthropozoonosis, environmental and foodstuff microbiology.
The expanding hazard of the multidrug-resistant microorganisms and the similar host immune reaction, the hot concepts for the therapy of biofilm-based, acute and persistent microbial infections, in addition to the advance of latest vaccines and extra efficacious antimicrobial medicinal drugs to avoid and deal with human and animal infections can be additionally reviewed during this sequence within the mild of the newest achievements in those fields.
Special realization may be dedicated to the quick diffusion all over the world of the recent findings of the main complex translational researches conducted within the assorted fields of microbiological sciences, with the purpose to advertise a steered validation and move at medical point of the main promising experimental results.
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Extra resources for Advances in Microbiology, Infectious Diseases and Public Health: Volume 1
Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) cell proliferation assay (ATCC, Manassas, USA), 36 V. Vastano et al. according to the manufacturer’s protocol. The culture media were then collected, cells were washed twice with phosphate-buffered saline (PBS) and harvested with High Pure RNA Isolation Kit (Roche Diagnostics, Milano, Italy) from Caco-2 cells infected or not with L. plantarum. 3 Real-Time PCR To study expression of TLR2, TLR4, HBDs, IL-6, TGF-β and IL-10 in Caco-2 cells, RealTime PCR was performed on RNA extracted from the cells challenged with L plantarum LM3 and LM3-2.
Toxicol In Vitro 18 (3):231–243 Werthe´n M, Henriksson L, Jensen PØ, Sternberg C, Givskov M, Bjarnsholt T (2010) An in vitro model of bacterial infections in wounds and other soft tissues. APMIS 118(2):156–164 White RJ, Cutting KF (2006) Critical colonization--the concept under scrutiny. Ostomy Wound Manage 52 (11):50–56 Wolcott RD, Rumbaugh KP, James G, Schultz G, Phillips P, Yang Q, Watters C, Stewart PS, Dowd SE (2010a) Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window.
Plantarum. 3 Real-Time PCR To study expression of TLR2, TLR4, HBDs, IL-6, TGF-β and IL-10 in Caco-2 cells, RealTime PCR was performed on RNA extracted from the cells challenged with L plantarum LM3 and LM3-2. 0 Instrument, GmbH, Mannheim, Germany) according to the manufacturer’s instructions. 5 μM of each primer and 1X LightCycler-FastStart DNA Master SYBR Green I mix. Sequences of the oligonucleotides used for amplification and related programs are shown in Table 1. 20 C/ s up to 95 C, with continuous measurement of fluorescence to confirm amplification of specific transcripts.
Advances in Microbiology, Infectious Diseases and Public Health: Volume 1 by Gianfranco Donelli (eds.)